Our paper on two-photon uncaging of IP3 in living cells came out today in ChemBioChem.
DOI: 10.1002/cbic.200500345
Our new cage (NV-IP3) was quite photosensitive as we used only 5 mW for 100 ms to produce calcium puffs or waves (we were surprised by how facile the uncaging was!). This is the first exampe of localised photolysis of IP3 using near-IR light (we used a mode-locked Ti:sapphire laser at 710 nm).
DOI: 10.1002/cbic.200500345
Our new cage (NV-IP3) was quite photosensitive as we used only 5 mW for 100 ms to produce calcium puffs or waves (we were surprised by how facile the uncaging was!). This is the first exampe of localised photolysis of IP3 using near-IR light (we used a mode-locked Ti:sapphire laser at 710 nm).
posted by graham at 4:10 PM
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