In vivo uncaging of calcium?
The Nedergaard lab have a paper coming out soon in Nature Neuroscience (published online: 25 December 2005; | doi:10.1038/nn1623) in which they cliam to use DM-nitrophen AM for UV uncaging of calcium in living mice. The images presented are quite beautiful, but the uncaging is impossible as DM-nitrophen has to be caged magnesium, as mentioned earlier on my weblog. They have no excuse, as Molecular Probes sell the probe as "DMNB-EDTA", since Calbiochem trademarked "DM-nitrophen" (somehow). It says EDTA in the name of the compound: has everyone forgotten in their rush to do biology, that this binds magnesium very well? That's why everyone uses the calcium selective probes EGTA or BAPTA for intracellular work.
The Nedergaard lab have a paper coming out soon in Nature Neuroscience (published online: 25 December 2005; | doi:10.1038/nn1623) in which they cliam to use DM-nitrophen AM for UV uncaging of calcium in living mice. The images presented are quite beautiful, but the uncaging is impossible as DM-nitrophen has to be caged magnesium, as mentioned earlier on my weblog. They have no excuse, as Molecular Probes sell the probe as "DMNB-EDTA", since Calbiochem trademarked "DM-nitrophen" (somehow). It says EDTA in the name of the compound: has everyone forgotten in their rush to do biology, that this binds magnesium very well? That's why everyone uses the calcium selective probes EGTA or BAPTA for intracellular work.
posted by graham at 4:14 PM
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