GFP strikes again.
Is there simply no end to what "GFP" can be made to do? The Russian group lead by Lukyanov have publshed another important paper in this field; this time they have made a mutant of DsRed which they call "Killer Red" (cool name!) that can be used for genetically targeted CALI. They find one set of mutants in their screening that micraculously produces a good deal of singlet oxygen. They tested many other RFPs and only KillerRed had this useful property. Probably the best expt they did was tad a PH domain with eGFP and KillerRed (or DsRed). The effects of irradiation of both constructs was strikingly different: only the former inactivated the PH domain, as shown by dissociation of the eGFP signal from the plasma membrane, and concomitant increase of cytosolic fluorescence.
(Nature Biotechnology (2006) 24: 95-99; doi:10.1038/nbt1175)
Is there simply no end to what "GFP" can be made to do? The Russian group lead by Lukyanov have publshed another important paper in this field; this time they have made a mutant of DsRed which they call "Killer Red" (cool name!) that can be used for genetically targeted CALI. They find one set of mutants in their screening that micraculously produces a good deal of singlet oxygen. They tested many other RFPs and only KillerRed had this useful property. Probably the best expt they did was tad a PH domain with eGFP and KillerRed (or DsRed). The effects of irradiation of both constructs was strikingly different: only the former inactivated the PH domain, as shown by dissociation of the eGFP signal from the plasma membrane, and concomitant increase of cytosolic fluorescence.
(Nature Biotechnology (2006) 24: 95-99; doi:10.1038/nbt1175)
posted by graham at 1:42 PM
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